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载柔红霉素膜微粒的制备及其对白血病细胞的增殖抑制作用
向虹霖, 王慧洁, 卿红, 周瑶, 毛声俊, 李慧
中国药学杂志 ›› 2018, Vol. 53 ›› Issue (7) : 526-530.
PDF(1788 KB)
PDF(1788 KB)
载柔红霉素膜微粒的制备及其对白血病细胞的增殖抑制作用
Preparation and Anti-Leukemia Efficacy of Daunorubicin-Loaded Microparticles
目的 构建载柔红霉素膜微粒给药系统,考察其对白血病细胞的增殖抑制作用。方法 采用胞内与胞外载药2种方式,制备来源于HL-60细胞的载柔红霉素膜微粒,并表征其粒径、Zeta电位和载药量等特性。采用激光共聚焦显微镜与流式细胞术检测细胞对载药膜微粒的摄取,MTT法检测载药膜微粒对肿瘤细胞的增殖抑制作用。结果 2种方式所制得膜微粒的粒径和Zeta电位无明显差异,而胞外载药膜微粒的载药量较高。细胞摄取试验结果显示,载药膜微粒可显著增加细胞摄取(P<0.05),且胞内载药较胞外载药方式更优(P<0.05)。MTT试验结果表明,与游离药物相比,载药膜微粒可增强其对HL-60细胞的增殖抑制作用(P<0.05)。结论 不同载药方式对膜微粒的抗肿瘤药效具有一定影响,膜微粒可显著增强细胞摄取与药效,有望成为具有潜力的治疗白血病的新型递药载体。
OBJECTIVE To construct a novel daunorubicin-loaded microparticles (DNR-MPs)drug delivery system, and study its proliferation inhibiton effect on leukemia cells. METHODS Both DNR-MPs-IDL(DNR microparticles with intracellular drug loading method)and DNR-MPs-EDL (DNR microparticles with extracellular drug loading method)were prepared from HL-60 cells, and the average particle size, Zeta potential and drug loading efficiency of DNR-MPs were measured. The uptake of DNR-MPs by HL-60 cells was analyzed by confocal laser scanning microscopy (CLSM)and flow cytometry (FCM). The inhibition effect of DNR-MPs on the proliferation of HL-60 cells was evaluated by MTT assay. RESULTS There was no significant difference in the average particle size and Zeta potential of the two DNR-MPs. The drug loading efficiency of DNR-MPs-EDL was higher compared with DNR-MPs-IDL. The uptake RESULTS showed that the two DNR-MPs significantly increased the drug uptake compared with free DNR (P<0.05). DNR-MPs-IDL showed a higher drug uptake by the cells than DNR-MPs-EDL did (P<0.05). They also exhibited an enhanced inhibition effect on the proliferation of HL-60 cells compared with DNR (P<0.05). CONCLUSION The different ways of preparation of drug-loaded microparticles can cause varying anti-tumor effect. Microparticles can significantly augment the anti-leukemia efficacy of DNR, indicating a promising drug carrier for the therapy of leukemia.
膜微粒 / 白血病 / 柔红霉素 {{custom_keyword}} /
microparticle / leukemia / daunorubicin {{custom_keyword}} /
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四川省科技厅项目资助(2014JY0100)
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